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c6 rat glial cells  (ATCC)


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    Structured Review

    ATCC c6 rat glial cells
    C6 Rat Glial Cells, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1969 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/c6+rat+glial+cells/C6/10__3390_slash_ph19040573-142-1-7
    Average 96 stars, based on 1969 article reviews
    c6 rat glial cells - by Bioz Stars, 2026-10
    96/100 stars

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    Related Articles

    Cell Culture:

    Article Title: Antiamoebic activities of flavonoids against pathogenic free-living amoebae, Naegleria fowleri and Acanthamoeba species
    Article Snippet: A. castellanii (ATCC-30868) and A. polyphaga (ATCC-30461) were axenically cultured and maintained in peptone-yeast-glucose medium supplemented with 1% penicillin/streptomycin at 25°C. .. C6 rat glial cells (ATCC CCL-107) were cultured in Dulbecco’s modified Eagle medium (Gibco) supplemented with 10% fetal bovine serum (Gibco) and 1% penicillin/streptomycin (Gibco) at 37°C in a humidified 5% CO2 incubator. .. Neglaria fowleri trophozoites (5× 104 cells/well) were seeded on 96-well microplates (Thermo Fisher Scientific, Waltham, MA, USA) in Nelson’s medium and incubated at 37°C overnight.

    Article Title: (‒)-Epicatechin reveals amoebicidal activity against Acanthamoeba castellanii by activating the programmed cell death pathway.
    Article Snippet: Background: Acanthamoeba is an opportunistic pathogen that can cause human infections such as granulomatous amebic encephalitis and acanthamoeba keratitis.. However, no specific drug to treat the diseases has been developed.. Therefore, the discovery or development of novel drugs for treating Acanthamoeba infections is urgently needed.

    Article Title: Discovery of Cyclopentane-Based Phospholipids as Miltefosine Analogs with Superior Potency and Enhanced Selectivity Against Naegleria fowleri
    Article Snippet: Amoeba trophozoites were cultured and maintained in Nelson’s medium containing 5% foetal bovine serum (FBS; Gibco, Grand Island, NY, USA) and 1% penicillin/streptomycin (P/S; Gibco, Grand Island, NY, USA) at 37 °C [ ]. .. C6 rat glial cells (C6 cells; ATCC CCL-107) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, Grand Island, NY, USA) supplemented with 10% FBS (Gibco, Grand Island, NY, USA) and 1% P/S (Gibco, Grand Island, NY, USA) at 37 °C in a humidified incubator under 5% CO 2 atmosphere. .. N. fowleri trophozoites (5 × 10 4 cells/well) were seeded in a 96-well microplate (Thermo Fisher Scientific, Waltham, MA, USA) in Nelson’s medium and incubated at 37 °C overnight.

    Article Title: Discovery of Cyclopentane-Based Phospholipids as Miltefosine Analogs with Superior Potency and Enhanced Selectivity Against Naegleria fowleri .
    Article Snippet: Amoeba trophozoites were cultured and maintained in Nelson’s medium containing 5% foetal bovine serum (FBS; Gibco, Grand Island, NY, USA) and 1% penicillin/streptomycin (P/S; Gibco, Grand Island, NY, USA) at 37 ◦C [56]. .. C6 rat glial cells (C6 cells; ATCC CCL-107) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, Grand Island, NY, USA) supplemented with 10% FBS (Gibco, Grand Island, NY, USA) and 1% P/S (Gibco, Grand Island, NY, USA) at 37 ◦C in a humidified incubator under 5% CO2 atmosphere. .. N. fowleri trophozoites (5 × 104 cells/well) were seeded in a 96-well microplate (Thermo Fisher Scientific, Waltham, MA, USA) in Nelson’s medium and incubated at 37 ◦C overnight.

    Article Title: Ginsenosides Rb3 and Rc Exhibit Anti-Amoebic Activities Against Naegleria fowleri, the Etiological Agent of Primary Amoebic Meningoencephalitis
    Article Snippet: N. fowleri (ATCC 30215, Carter NF69 strain; American Type Culture Collection, Manassas, VA, USA) was axenically cultured in Nelson’s medium, containing 1% penicillin/streptomycin (P/S; Gibco, Grand Island, NY, USA) and 5% fetal bovine serum (FBS; Gibco). .. The C6 rat glial cells (C6 cells; ATCC CCL-107) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco), supplemented with 1% P/S (Gibco) and 10% FBS (Gibco). ..

    Article Title: Optimization of Culture Conditions for High Cell Productivity and Astaxanthin Accumulation in Vietnam’s Green Microalgae Haematococcus pluvialis HB and a Neuroprotective Activity of Its Astaxanthin
    Article Snippet: .. C6 rat glial cells (ATCC, CCL-107TM) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Merck, Darmstadt, Germany)/high glucose, supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, at 37 °C with 5% CO 2 . ..

    Modification:

    Article Title: Antiamoebic activities of flavonoids against pathogenic free-living amoebae, Naegleria fowleri and Acanthamoeba species
    Article Snippet: A. castellanii (ATCC-30868) and A. polyphaga (ATCC-30461) were axenically cultured and maintained in peptone-yeast-glucose medium supplemented with 1% penicillin/streptomycin at 25°C. .. C6 rat glial cells (ATCC CCL-107) were cultured in Dulbecco’s modified Eagle medium (Gibco) supplemented with 10% fetal bovine serum (Gibco) and 1% penicillin/streptomycin (Gibco) at 37°C in a humidified 5% CO2 incubator. .. Neglaria fowleri trophozoites (5× 104 cells/well) were seeded on 96-well microplates (Thermo Fisher Scientific, Waltham, MA, USA) in Nelson’s medium and incubated at 37°C overnight.

    Article Title: Naegleria fowleri Extracellular Vesicles Induce Proinflammatory Immune Responses in BV-2 Microglial Cells
    Article Snippet: .. BV-2 mouse microglial cells and C6 rat glial cells (ATCC CCL-107) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM; Welgene, Daegu, Republic of Korea) supplement with 10% FBS (Gibco, Grand Island, NY, USA) and 1% P/S (Gibco, Grand Island, NY, USA). ..

    Article Title: Discovery of Cyclopentane-Based Phospholipids as Miltefosine Analogs with Superior Potency and Enhanced Selectivity Against Naegleria fowleri
    Article Snippet: Amoeba trophozoites were cultured and maintained in Nelson’s medium containing 5% foetal bovine serum (FBS; Gibco, Grand Island, NY, USA) and 1% penicillin/streptomycin (P/S; Gibco, Grand Island, NY, USA) at 37 °C [ ]. .. C6 rat glial cells (C6 cells; ATCC CCL-107) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, Grand Island, NY, USA) supplemented with 10% FBS (Gibco, Grand Island, NY, USA) and 1% P/S (Gibco, Grand Island, NY, USA) at 37 °C in a humidified incubator under 5% CO 2 atmosphere. .. N. fowleri trophozoites (5 × 10 4 cells/well) were seeded in a 96-well microplate (Thermo Fisher Scientific, Waltham, MA, USA) in Nelson’s medium and incubated at 37 °C overnight.

    Article Title: Discovery of Cyclopentane-Based Phospholipids as Miltefosine Analogs with Superior Potency and Enhanced Selectivity Against Naegleria fowleri .
    Article Snippet: Amoeba trophozoites were cultured and maintained in Nelson’s medium containing 5% foetal bovine serum (FBS; Gibco, Grand Island, NY, USA) and 1% penicillin/streptomycin (P/S; Gibco, Grand Island, NY, USA) at 37 ◦C [56]. .. C6 rat glial cells (C6 cells; ATCC CCL-107) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, Grand Island, NY, USA) supplemented with 10% FBS (Gibco, Grand Island, NY, USA) and 1% P/S (Gibco, Grand Island, NY, USA) at 37 ◦C in a humidified incubator under 5% CO2 atmosphere. .. N. fowleri trophozoites (5 × 104 cells/well) were seeded in a 96-well microplate (Thermo Fisher Scientific, Waltham, MA, USA) in Nelson’s medium and incubated at 37 ◦C overnight.

    Article Title: Naegleria fowleri Extracellular Vesicles Induce Proinflammatory Immune Responses in BV-2 Microglial Cells.
    Article Snippet: .. BV-2 mouse microglial cells and C6 rat glial cells (ATCC CCL-107) were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM; Welgene, Daegu, Republic of Korea) supplement with 10% FBS (Gibco, Grand Island, NY, USA) and 1% P/S (Gibco, Grand Island, NY, USA). ..

    Article Title: Ginsenosides Rb3 and Rc Exhibit Anti-Amoebic Activities Against Naegleria fowleri, the Etiological Agent of Primary Amoebic Meningoencephalitis
    Article Snippet: N. fowleri (ATCC 30215, Carter NF69 strain; American Type Culture Collection, Manassas, VA, USA) was axenically cultured in Nelson’s medium, containing 1% penicillin/streptomycin (P/S; Gibco, Grand Island, NY, USA) and 5% fetal bovine serum (FBS; Gibco). .. The C6 rat glial cells (C6 cells; ATCC CCL-107) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco), supplemented with 1% P/S (Gibco) and 10% FBS (Gibco). ..

    Article Title: Optimization of Culture Conditions for High Cell Productivity and Astaxanthin Accumulation in Vietnam’s Green Microalgae Haematococcus pluvialis HB and a Neuroprotective Activity of Its Astaxanthin
    Article Snippet: .. C6 rat glial cells (ATCC, CCL-107TM) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) (Merck, Darmstadt, Germany)/high glucose, supplemented with 10% fetal bovine serum (FBS) and 1% penicillin/streptomycin, at 37 °C with 5% CO 2 . ..



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    Specific binding and quantification of dye–labeled aptamers in U87MG (human astrocytic <t>glioblastoma)</t> and <t>C6</t> (rat glial glioblastoma) cells. (A, B) Representative FL images of U87MG and C6 cells treated with Alexa488–labeled ΔAst17-30 (200 nM) or Tri-ΔAst17-30 (66 nM) aptamers, showing BF, Alexa488 (green), DAPI (blue), and merged channels. Scale bars, 100 μm. (C) Box plots of MFI values of the two aptamers showing significant differences in aptamer binding between U87MG and C6 cells ( p < 0.0001 for both ΔAst17-30 and Tri-ΔAst17-30, N = 12), as well as significant difference in binding strength between the two aptamers in U87MG cells ( p < 0.0001, N = 12). (D) Flow cytometry quantifies Alexa488–positive U87MG and C6 cells after Tri-ΔAst17-30 treatment (final 66 nM), revealing specific aptamer binding in U87MG but not C6 cells. The square gates define the percentage of aptamer-positive cells within the total population, which was 92.9 % for U87MG and 4.73 % for C6, respectively. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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    Specific binding and quantification of dye–labeled aptamers in U87MG (human astrocytic <t>glioblastoma)</t> and <t>C6</t> (rat glial glioblastoma) cells. (A, B) Representative FL images of U87MG and C6 cells treated with Alexa488–labeled ΔAst17-30 (200 nM) or Tri-ΔAst17-30 (66 nM) aptamers, showing BF, Alexa488 (green), DAPI (blue), and merged channels. Scale bars, 100 μm. (C) Box plots of MFI values of the two aptamers showing significant differences in aptamer binding between U87MG and C6 cells ( p < 0.0001 for both ΔAst17-30 and Tri-ΔAst17-30, N = 12), as well as significant difference in binding strength between the two aptamers in U87MG cells ( p < 0.0001, N = 12). (D) Flow cytometry quantifies Alexa488–positive U87MG and C6 cells after Tri-ΔAst17-30 treatment (final 66 nM), revealing specific aptamer binding in U87MG but not C6 cells. The square gates define the percentage of aptamer-positive cells within the total population, which was 92.9 % for U87MG and 4.73 % for C6, respectively. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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    ATCC rat c6 glial cells
    Specific binding and quantification of dye–labeled aptamers in U87MG (human astrocytic <t>glioblastoma)</t> and <t>C6</t> (rat glial glioblastoma) cells. (A, B) Representative FL images of U87MG and C6 cells treated with Alexa488–labeled ΔAst17-30 (200 nM) or Tri-ΔAst17-30 (66 nM) aptamers, showing BF, Alexa488 (green), DAPI (blue), and merged channels. Scale bars, 100 μm. (C) Box plots of MFI values of the two aptamers showing significant differences in aptamer binding between U87MG and C6 cells ( p < 0.0001 for both ΔAst17-30 and Tri-ΔAst17-30, N = 12), as well as significant difference in binding strength between the two aptamers in U87MG cells ( p < 0.0001, N = 12). (D) Flow cytometry quantifies Alexa488–positive U87MG and C6 cells after Tri-ΔAst17-30 treatment (final 66 nM), revealing specific aptamer binding in U87MG but not C6 cells. The square gates define the percentage of aptamer-positive cells within the total population, which was 92.9 % for U87MG and 4.73 % for C6, respectively. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)
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    Image Search Results


    Specific binding and quantification of dye–labeled aptamers in U87MG (human astrocytic glioblastoma) and C6 (rat glial glioblastoma) cells. (A, B) Representative FL images of U87MG and C6 cells treated with Alexa488–labeled ΔAst17-30 (200 nM) or Tri-ΔAst17-30 (66 nM) aptamers, showing BF, Alexa488 (green), DAPI (blue), and merged channels. Scale bars, 100 μm. (C) Box plots of MFI values of the two aptamers showing significant differences in aptamer binding between U87MG and C6 cells ( p < 0.0001 for both ΔAst17-30 and Tri-ΔAst17-30, N = 12), as well as significant difference in binding strength between the two aptamers in U87MG cells ( p < 0.0001, N = 12). (D) Flow cytometry quantifies Alexa488–positive U87MG and C6 cells after Tri-ΔAst17-30 treatment (final 66 nM), revealing specific aptamer binding in U87MG but not C6 cells. The square gates define the percentage of aptamer-positive cells within the total population, which was 92.9 % for U87MG and 4.73 % for C6, respectively. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Journal: Materials Today Bio

    Article Title: Y-shaped trivalent aptamer for targeted visualization and tracking of reprogrammed astrocytes

    doi: 10.1016/j.mtbio.2025.102482

    Figure Lengend Snippet: Specific binding and quantification of dye–labeled aptamers in U87MG (human astrocytic glioblastoma) and C6 (rat glial glioblastoma) cells. (A, B) Representative FL images of U87MG and C6 cells treated with Alexa488–labeled ΔAst17-30 (200 nM) or Tri-ΔAst17-30 (66 nM) aptamers, showing BF, Alexa488 (green), DAPI (blue), and merged channels. Scale bars, 100 μm. (C) Box plots of MFI values of the two aptamers showing significant differences in aptamer binding between U87MG and C6 cells ( p < 0.0001 for both ΔAst17-30 and Tri-ΔAst17-30, N = 12), as well as significant difference in binding strength between the two aptamers in U87MG cells ( p < 0.0001, N = 12). (D) Flow cytometry quantifies Alexa488–positive U87MG and C6 cells after Tri-ΔAst17-30 treatment (final 66 nM), revealing specific aptamer binding in U87MG but not C6 cells. The square gates define the percentage of aptamer-positive cells within the total population, which was 92.9 % for U87MG and 4.73 % for C6, respectively. (For interpretation of the references to color in this figure legend, the reader is referred to the Web version of this article.)

    Article Snippet: Human astrocytic glioblastoma U-87MG and rat glial glioblastoma C6 cell lines were acquired form the Korean Cell Line Bank (Seoul, Korea).

    Techniques: Binding Assay, Labeling, Flow Cytometry